Prevalence and clinical significance of FLT3 internal tandem duplication mutation in acute myeloid leukemia of older people
10.3760/cma.j.issn.1009-9921.2009.04.011
- VernacularTitle:老年急性髓细胞白血病患者FLT3-ITD基因突变的临床意义
- Author:
Ye WANG
;
Liefen YIN
;
Xiaoyuan QU
;
Bo PENG
;
Lifang CHENG
;
Ling YANG
- Publication Type:Journal Article
- Keywords:
Leukemia,myeloid,acute;
Fms-like tyrosine kinase 3;
Tandem repeat sequences;
Polymerase chain reaction;
Chromatography,high pressure liquid
- From:
Journal of Leukemia & Lymphoma
2009;18(4):220-222
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of the study was to detect the mutation of Fms-like tyrosine kinase 3 internal tandem duplication (FLT3-1TD) rate in older de novo acute myeloid leukemia (AML) patients, to evaluate the role of FLT3-ITD in AML and its clinical significance. Methods The mutations of FLT3-1TD in bone marrow mononuelear cells (MNCS) from 30 cases of older AML were screened by polymerase chain reaction denaturing-high performance liquid chromatography (PCR-DHPLC). Results FLT3-1TD mutations were identified in 26.67 %(8/30) patients, while there were no mutations identified in control cases. And these kinds of mutations were likely to attend in M3 types. All mutations of FLT3-ITD were heterozygous and rearrangement fragment located in reading frame. Different karyomite groups had different FLT3-ITDmutations rate. We could see that FLT3-ITD positive patients were more prevalent in patients with normal karyotype. Clinical researches indicated that FLT3-ITD mutations had the characteristics of a higher peripheral white cell count, higher blast cells and lower complete remission rate in older AMKA Conclusion FLT3-ITD positive older AML patients conferred a poor prognosis and were likely to attend in normal karyomite group. The detection of FLT3-ITD mutations could make up for the deficiency of cytogenetics to some extent, and may become a routine examination of AML in older, which can direct their treatment and predict their prognosis.
