Effect of anti-apoptotic protein HSP-90 in human multiple myeloma cell line U266 cells with bortezomib
10.3760/cma.j.issn.1009-9921.2010.03.012
- VernacularTitle:硼替佐米对人类多发性骨髓瘤细胞系U266细胞抗凋亡蛋白HSP-90的影响
- Author:
Lingjuan HUANG
;
Yanping MA
;
Yirong YANG
;
Linhua YANG
- Publication Type:Journal Article
- Keywords:
Neoplasms,experimental;
Multiple myeloma;
Cell line,tumor,U266;
HSP-90 heat-shock proteins
- From:
Journal of Leukemia & Lymphoma
2010;19(3):166-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of anti-apoptotic protein HSP-90 in human multiple myeloma cell line U266 cells after bertezomib interaction. Methods The HSP-90 mRNA expression in the U266 cells was tested by reverse transcription polymerase chain reaction (RT-PCR) after 4 hours of treatment of bertezomib by different concentration. Results With the of increased concentration of bortezomib, the expression level of HSP-90 αmRNA was also increased in U266 cells. Respectively, quantitative results of HSP-90α are 0.343±0.017, 0.505±0.039, 0.640±0.029, 0.760±0.059, 0.963±0.054 from the low to high concentration of bertezomib groups. And there are statistical difference between each group(P <0.05). However, the HSP-90β quantitative results in 0 nmol/L concentration of bertezomib (0.61±0.022) have statistical difference between 50, 150, 200 nmol/L groups(P <0.05). HSP-90β quantitative results in 50(0.765±0.050)and 100 nmol/L(0.645±0.052) nmol/L groups are different(P <0.05). Compared with 100 nmol/L concentration of bortezomib group, statistical difference also exists in 150 (0.770±0.059) and 200 nmol/L (0.790±0.027)groups (P <0.05). Although there is no obvious increase in the mRNA expression of HSP-90β from the chart, statistical difference existed in the whole data (P <0.05). Conclusion Bortezomib can increase the level expression of HSP-90 mRNA, and especially increase the level expression of HSP-90α mRNA.
