Ultrasound-targeted microbubble mediated liposome small interference RNA transfection to retinal pigment epithelium cells
- VernacularTitle:超声辐照微泡介导脂质体小干扰RNA转染视网膜色素上皮细胞
- Author:
Hongli LI
;
Lianfang DU
;
Xiaozhi ZHENG
;
Huiping WANG
;
Qing GU
;
Xiafang CHEN
- Publication Type:Journal Article
- Keywords:
Ultrasound;
Microbubble;
Small interference RNA;
Retinal pigment epithelium cells
- From:Chinese Journal of Medical Imaging Technology
2010;26(1):22-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the transfection efficiency of combination of ultrasound (US) microbubbles (MBs) mediated liposome small interference RNA (siRNA) to human and rat retinal pigment epithelium (RPE) cells. Methods Human and rat RPE cells and siRNA were incubated in 24-well plates (2×10~5/well and 1×10~5/well, respectively). The cells were devided into 5 groups: siRNA+US, siRNA+MBs+US, siRNA+L (liposome), siRNA+L+US, siRNA+L+US+MBs. After 12 h, transfection efficiency was observed with fluorescence microscopy and flow cytometry. Results US or ultrasound targeted microbubbles destruction without liposome-mediated could not promote siRNA transfection efficiency to human and rat RPE cells. The transfection efficiency of human and rat RPE cells significantly decreased in the siRNA+L+US+MBs group, but increased in siRNA+L+US group. Conclusion Ultrasonic irradiation can promote lipid-mediated siRNA transfected human RPE cells.
