Expression of MCP-1and its receptor CCR2 in tumor cells and stromal cells of multiple myeloma
10.3760/cma.j.issn.1009-9921.2012.07.006
- VernacularTitle:单个核细胞趋化蛋白-1及其受体CCR2在多发性骨髓瘤基质细胞与瘤细胞中的表达
- Author:
Yuejie GUO
;
Weihua ZHANG
;
Xiulian ZHANG
;
Xinghuo FAN
;
Jie TAO
;
Fang WEI
;
Suming HOU
;
Wei CHEN
- Publication Type:Journal Article
- Keywords:
Multiple myeloma;
MCP-1;
CCR2;
Flow cytometry
- From:
Journal of Leukemia & Lymphoma
2012;21(7):405-407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of chemokine (MCP-1) and chemokine receptor (CCR2) in bone marrow cells,bone marrow stromal cells of multiple myeloma (MM) patients.Methods 15 cases were diagnosed by domestic uniform standard for MM patients,7 cases of male,8 cases of female,age range from 38 to 67 years,mean age 53.7 years old.According to the Durie-Salmon staging system,patients were divided into Ⅰ (2 cases),Ⅱ (5 cases) and Ⅲ period(8 cases).Control group were from 10 cases of non-malignant blood disease patients.MCP-1,CCR2 expression were measured by flow cytometry.Results Almost 14 cases of bone marrow cells expressed MCP-1and CCR2 in MM patients,while in the control group,bone marrow cells almost did not express MCP-1and CCR2.Stromal cells had similar MCP-1and CCR2 expression profile (68.17 ％ vs 4.27 ％. P＜0.05).Tumor cells of MCP-1/CCR2 expression rates were 3.25 ％ and 32.76 ％. Compared MCP-1/ CCR2 expression of stromal cells and tumor cells with different stages of disease, the activated stage and the stable stage had similar level (68.71％ and 32.76 ％ vs 70.12 ％ and 53.39 ％. P＞0.05). Conclusion Most patients with MM bone marrow were expressed MCP-1and CCR2.MCP-1and CCR2 are the major MM cell surface expression of chemokine/receptor, which play important roles in the progress of.
