Study on the three-dimensional chitosan-decellularised-derma scaffolds for bone tissue engineering
- VernacularTitle:壳聚糖-脱细胞真皮三维材料作为骨组织工程支架材料的研究
- Author:
Yan ZHANG
;
Ruixin LI
;
Xizheng ZHANG
;
Yong GUO
- Publication Type:Journal Article
- Keywords:
Chitosan;
Decellularised-derma Porosity;
Scanning electron microscopy;
Bone tissue engineering;
Scaffolds
- From:
Journal of Medical Biomechanics
2010;25(1):11-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the adhesion of MC3T3-EI osteoblastic progenitor cells to the three-dimensional chitosan-decellularised-derma scaffolds,and evaluate the cytocompatibility of the scaffolds.Method The threedimensional chitosan-decellularised-derma scaffolds were prepared by the freeze-dtying method,the porosity,density and water absorption of which were measured.The microscopic morphology of the composite scaffolds was analyzed by the scanning electron microscopy(SEM).The MC3T3-E1 cells cultivated in vitro were seeded onto the composite scaffolds,and then co-cultured for 2,3,4 and 5 hours.At each time point,three specimens from each matrix were taken to determine the cell-adhesion rate and the best time of the cell-adhesion.The cells were seeded onto the composite scaffolds,and then co-cultured for 1,3,5,7,9,11 and 13 days.The MC3T3-E1 cells inside were evaluated with MTS test.The cell morphology was observed by the histological staining.The compression tests were performed using a Universal Testing Machine,at room temperature,as compared with no-cell-scaffolds.Results The three-dimensional chitosan-decellularised-derma scaffolds have high interval poroslty with the porosity(92.8%),the density(0.09796 g/ml)and the water absorption(2169±100)%.The cytocompatibility test shows that the seeded MC3T3-E1 cells can adhere to the scaffolds and proliferate.Conclusions The three-dimensional chitosan-decellularised-derma scaffolds have high interval porosity with the welldistributed diameter.The MC3T3-E1 cells are easy to adhere the scaffolds and proliferate which shows that the scaffolds have a good cytocompatibility.
