Differential expression of genes in dendritic cell induced by human alloantigens and cytomegalovirus antigen
10.3760/cma.j.issn.1009-9921.2012.06.003
- VernacularTitle:同种异体抗原和巨细胞病毒抗原诱导的突状细胞基因表达差异
- Author:
Xiuping ZOU
;
Wangbing CHEN
;
Shanshan LIU
;
Wei HUANG
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Alloantigens;
Cytomegalovirus antigen;
Acute graft-versus-host disease;
Oligonucleotide array sequence analysis
- From:
Journal of Leukemia & Lymphoma
2012;21(6):330-334
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate differential gene expression in dendritic cell(DC) in response to human alloantigen and cytomegalovirus protein,and search target genes which can prevent graft rejection and eytomegalovirus (CMV) infection.[Methods]Genome-wide microarray analysis was performed to test gene expression in DC in response to human alloantigen and cytomegalovirus protein 3A(CMV3A).[Results]The Results showed that the difference of gene expression of DC induced by CMV3A and alloantigen was significant.The genes with differential expression included antigen processing and presentation,toll-like receptor signaling pathway and cell movement/migration, cytokine-cytokine receptor interaction and metabolism of xenobiotics by cytochrome p450,and several heat shock protein(HSP) family members including HSPA5,HSPA8,HSPA9B and HSP90AB1,in DC induced hy alloantigen were higher expression than that by CMV3A.[Conclusion]This study found several genes including heat shock protein family in DC induced by alloantigen were higher expression than that by CMV3A,these genes might play a valuable role in preventing graft rejection and CMV infection.
