Study on immunophenotyping with multi-color flow cytometry in adult patients with acute myeloid leukemia
10.3760/cma.j.issn.1009-9921.2009.05.005
- VernacularTitle:多参数流式细胞术在成年人急性髓系白血病免疫分型中的应用
- Author:
Xiaodong XU
;
Shengming ZHAO
;
Tianlin ZHENG
;
Naibai CHANG
- Publication Type:Journal Article
- Keywords:
Leukemia,myeloid;
Flow cytometry;
Immunophenotyping
- From:
Journal of Leukemia & Lymphoma
2009;18(5):270-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the characteristics of immunophenotyping in adults with acute myeloid leukemia (AML) using muhi-eolor flow cytometry. Methods Immunophenotyping was performed by three color flow cytometry using CD45/SSC gating. Results In 126 patients with AML, the myeloid antigen of CD13, CD33 and CD117 was highly expressed. The positive rate was 86.4 %, 70.2 % and 90.4 %, respectively. The CD34 and HLA-DR were lowerly expressed as 63.5 % and 61.7 %, respectively. About 34.2 % of lymphoid-assoeiated antigen expression of all the AML patients. The lymphoid-associated antigens of CD7 and CD19 expression in patients with AML was 23.6 % and 2.3 %, respectively. Conclusion Multi-color flow cytometry is an important method for diagnosis and prognosis for AML.
