Effect of Serum from Maintenance Hemodialysis Patients on Expression of ICAM-1 in Human Umbilical Vein Endothelial Cells
10.3969/j.issn.0253-9896.2010.01.006
- VernacularTitle:血透患者血清对人脐静脉血管内皮细胞ICAM-1表达的影响
- Author:
Wenhui YU
;
Xuena GUO
;
Guijiang SUN
;
Haibo YU
;
Hong DONG
;
Yitao LIU
- Publication Type:Journal Article
- Keywords:
renal dialysis;
serum;
umbilical veins;
endothelial cells;
cell adhesion molecules
- From:
Tianjin Medical Journal
2010;38(1):17-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of serum from maintenance hemodialysis(MHD) patients on the expression of intercellular adhesion molecule-1(ICAM-1) in human umbilical vein endothelial cells(HUVEC) cultured in vitro, and the mechanism of endothelial cells dysfunction caused by serum from MHD patients thereof. Methods: HUVEC were incubated for 1 h, 3 h, 6 h and 12 h in RPMI 1640 culture media containing 10 % fetal cattle serum(FCS )(group A), 10% normal human serum (group B) and 10% MHD patient serum (group C) respectively. The immunocytochemical method was used to determine the expression of ICAM-1. Results: There were significant differences in the expression of ICAM-1 in HUVEC among stimulation groups with different serums(P < 0.05). Compared with groups A and B, the expression of ICAM-1 was significantly increased in HUVEC of group C. There was significant difference in the expression of ICAM-1 at the different stimulation times(P < 0.05). There was significant interaction between the expression of ICAM-1 in HUVEC and stimulation groups with different serum and the stimulation times(P < 0.05). Conclusion: The serum from MHD patients with chronic renal failure can promote the expression of ICAM-1 in HUVEC cultured in vitro, thus causing the dysfunction of endothelial cell.
