Expression of FBXW7 in a murine model of T-cell acute lymphoblastic leukemia
10.3760/cma.j.issn.1009-9921.2011.12.003
- VernacularTitle:FBXW7在小鼠急性T淋巴细胞白血病模型中的表达
- Author:
Jia WEI
;
Chen TIAN
;
Jinghui CHONG
;
Cuihua MA
;
Yongmin LIN
;
Guoguang ZHENG
- Publication Type:Journal Article
- Keywords:
Ligases;
FBXW7;
Gene expression;
Leukemia,T-cell
- From:
Journal of Leukemia & Lymphoma
2011;20(12):709-711
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of FBXW7 during the development of Notch1induced murine leukemia.Methods Notch1 over-expressing murine model of T-cell acute lymphoblastic leukemia was used to study the expression of FBXW7 by real-time PCR methods.Bone marrow mononuclear cells (BMNC) were isolated on different days after transplantation and CD45.2+ GFP+ leukemia cells were sorted by flow cytometry at late stage.The expression changes of FBXW7 were tested by real-time PCR.Results The mouse bone marrow cells both from leukemia and control groups expressed FBXW7.Different expression patterns of FBXW7 were observed during the development of leukemia. The expression of FBXW7 was gradually increased in control group, whereas the expression level of FBXW7 in leukemia group was decreased steadily and reached one-sixth of that in control group on 12th day.Furthermore,lower expression level of FBXW7 was observed in CD45+.2 GFP+ leukemia cells.Conclusion Decreased expression of FBXW7 is observed in Notch1-induced mouse leukemia model,suggesting that the abnormal ubiquitin degradation pathway mediated by FBXW7 might contribute to the leukemogenesis in Notch1-induced murine leukemia model.
