Frequent epigenetic silencing of proapoptotic gene MAPK10 by methylation in B cell lymphoma

VernacularTitle:B细胞淋巴瘤中促凋亡基因MAPK10异常甲基化的研究
Author: Jianming YING ; Zifen GAO
Publication Type:Journal Article
Keywords: MAPK10; Methylation; Lymphoma,B-cell; Biomarker
From: Journal of Leukemia & Lymphoma 2010;19(5):272-275
CountryChina
Language:Chinese
Abstract: Objective To investigate epigenetic silencing of the proapoptotic gene MAPK10 by methylation in B-cell lymphoma. Methods We examined MAPK10 expression and methylation in seven cell lines derived from B-cell lymphoma by semi-quantitative RT-PCR and methylation-specific PCR (MSP), respectively. Methylation status was further examined in 24 diffuse large B-cell lymphoma (DLBCL), 15 follicular lymphoma (FL) and 12 reactive hyperplasia lymph nodes (LRH) both by MSP and bisulfite genomic sequencing (BGS). Results MAPK10 is silenced or downregulated in all seven B-cell lymphoma cell lines mostly due to promoter methylation. MAPK10 methylation was frequently detected in DLBCL (17 of 24, 71%) and FL (15 of 15, 100 %), but not in 12 LRH tissues. Conclusion MAPK10 is frequently inactivated by tumor-specific methylation, and thus, is a potential biomarker.