Safety and effect of umbilical cord blood-derived mesenchymal stem cells on apoptosis of human cardiomyocytes
- Author:
Shuixiang YANG
;
Jingling HUANG
- Publication Type:Journal Article
- Keywords:
Umbilical cord blood;
mesenchymal stem cells;
5-azaserine;
human cardiac myocyte;
apoptosis
- From:
Journal of Geriatric Cardiology
2010;07(2):110-115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the safety and effect of the umbilical cord blood(UCB)-derived mesenchymal stem cells(MSCs)on apoptosis of human cardiomyocytes(HCM). MethodsUCB was collected at the time of delivery with informed consent obtained from 10donors.The UCB-derived MSCs were treated with 5-azaserube(5-AZA)and were further induced to differentiate into cardiomyocytes.Telomerase activity,G-banding patterns of chromosomal karyotypes,tumor formation in nude mice,RT-PCR,and the effect of inhibiting apoptosis of HCM were investigated. ResultsMSCs derived from UCB were differentiated into cardiomyocytes in vitro,which possessed telomerase activity after 5-AZA induction,and no abnormal chromosomal karyotypes were observed.Expression of p53,cyclin A,cdk2,β-actin,C-fos,h-TERT and c-myc were similar in MSCs before and after 5-AZA treatment.There was no tumor formation in nude mice after injection of UCB-derived MSCs.UCB-derived MSCs significantly inhibited apoptosis of HCM. ConclusionUCB-derived MSCs are a valuable,safe and effective source of cell-transplantation treatment.
