Expression of E2F3 in Tissue of Bladder Transitional Cell Carcinoma and the Relationship with Clinical Significances
10.3969/j.issn.0253-9896.2010.01.003
- VernacularTitle:E2F3在膀胱癌组织中的表达及其与肿瘤临床特征的关系
- Author:
Hailong HU
;
Changli WU
;
Yan SUN
;
Wenlan ZHANG
;
Ruifa HAN
- Publication Type:Journal Article
- Keywords:
urinary bladder neoplasms;
carcinoma;
transitional cell;
transcription factors;
gene expression;
pathology;
clinical;
immunohistochemistry;
reverse transcriptase polymerase chain reaction
- From:
Tianjin Medical Journal
2010;38(1):7-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To acquire the expression of E2F3 protein and mRNA in bladder transitional cell carcinoma (BTCC) tissue and normal bladder epithelial tissue, and the relationship between E2F3 expression and the biological behaviors of BTCC thereof. Methods: Immunohistochemistry was used to detect the expression of E2F3 in BTCC(n = 64) and normal bladder mucosa(n = 10). Immunohistochemistry result was analysed by Image-pro Plus software and the expression result was indicated by integrated optical density (IOD). The expression of E2F3 mRNA was investigated using RT-PCR analysis in fresh bladder tumor tissues and normal bladder mucosa. Results: The expression rate of E2F3 in BTCC (32.8%) was higher than that of normal bladder mucosa(P < 0.01). The expression rate of E2F3 was strongly correlated with the pathological grade and clinical stage (P < 0.05;P < 0.01). Immunohistochemistry result indicated that the IOD of E2F3 was significantly higher in BTCC than that of normal bladder mucosa (P < 0.01). The expression level of E2F3 was strongly correlated with pathological grade (P < 0.01). Conclusion: E2F3 was the diagnostic and prognostic index of BTCC, and provided theory basis about the gene target therapy in BTCC.
