Effect of p38 MAPK pathway inhibitors SB203580 on cell cycle of leukemia K562 cell lines and its mechanisms

VernacularTitle:p38丝裂原活化蛋白激酶阻滞剂SB203580对白血病K562细胞周期的作用及机制
Author: Xiao GUO ; Chunjie DONG ; Dan SONG ; Wenjing LI ; Ling PAN
Publication Type:Journal Article
Keywords: Leukemia; Cell cycle; p38 mitogen-activated protein kinases; K562 cells; SB203580
From: Journal of Leukemia & Lymphoma 2009;18(8):449-451
CountryChina
Language:Chinese
Abstract: Objective To study the effect of p38 mitogen activated protein kinase (MAPK) pathway inhibitors SB203580 on cell cycle of K562 cell lines and its mechanisms. Methods The expression of mRNA and protein of p38,Cyclin D2,Cyelin E and P27 in K562 cell lines treated with SB203580 were detected by retrotranscription polymerase chain reaction (RT-PCR) and Western blotting, respectively. Cell cycle was determined by flow eytometry (FCM). Results The expressions of mRNA and protein of p38, Cyclin D2 and Cyclin E in K562 cell lines treated with SB203580 were decreased and the expression of p27 was increased.The percentage of cells in G0/G1 phase was increased and was decreased in S phase. There was a significant difference as compared with K562 cell lines before treated with SB203580. Conclusion SB203580 can affect cell cycle regulatory proteins by p38 pathway and eventually inhibit proliferation of K562 cells.