Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein
10.1016/j.jpha.2011.10.001
- Author:
Qianyun ZHANG
;
Hui CHEN
;
Zhen LIN
;
Jinming LIN
- Publication Type:Journal Article
- Keywords:
α-Fetoprotein;
Hepatocellular carcinoma;
Chemiluminescence enzyme immunoassay;
Magnetic microparticles;
Colorimetric enzyme-linked immunosorbent assay
- From:
Journal of Pharmaceutical Analysis
2012;02(2):130-135
- CountryChina
- Language:Chinese
-
Abstract:
A chemiluminescence enzyme immunoassay based on magnetic microparicles (MmPsCLEIA) was developed to evaluate serum α-fetoprotein (AFP) in parallel with tramional colorimetric enzyme-linked immunsorbrnt assay (ELISA).A sestematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer of immunoreagents,less total assay time,and better linearity,recovery,precision,senitivity and validity.AFP was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA,and the results werecompared with commercial electrochemilunminescence immunoassay (ECLIA) kit.The correlation coefficient between MPs-CLEIA and ELISA was obtained with R2=0.6703; however,the correlation between MPs-CLEIA and ECLIA (R2=0.9582) was obviously better than that between colorimetric ELISA and ECLIA (R2=0.6866).
