Inhibitation of effective bcl-2 siRNA on apoptosis of human leukemia-60 cells
10.3760/cma.j.issn.1009-9921.2009.12.002
- VernacularTitle:有效bcl-2 siRNA对HL-60细胞生长的抑制作用
- Author:
Chunyan YAN
;
Qingyuan YANG
;
Hong WEI
;
Xiaoyong LEI
;
Yulin TU
;
Xu WANG
;
Wen CUI
;
Lingling KONG
- Publication Type:Journal Article
- Keywords:
RNA interference;
Genes;
bcl-2;
RNA;
double-stranded;
HL-60 cells;
Apoptosis
- From:
Journal of Leukemia & Lymphoma
2009;18(12):712-713
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of bel-2 siRNA on apoptosis of HL-60 cells.Methods bcl-2 siRNA was synthesized in vitro transcription with silencer siRNA construction kit.The synthesized siRNA was transfected into HL-60 cells with Amine siPORT transfection.We used MTT flow cytometer and hoechst 33258 flourescence stainning t0 evaluate cell proliferation and apoptosis. Results.Bcl-2 siRNA could partially inhibit the growth of HL-60 cells.After incubated with bcl-2 siRNAl for 48 hours,HL-60 cells exhibited morphologic characteristic of apoptosis including chromatin condensation,crescents formation and nuclear fragmentation.Conclusion Effective bcl-2 siRNA can induce apoptosis and inhibit cell proliferation.
