Identification of TEL-AML1 fusion gene in childhood acute lymphoblastic leukemia
10.3760/cma.j.issn.1009-9921.2009.11.012
- VernacularTitle:儿童急性淋巴细胞白血病TEL-AML1融合基因检测
- Author:
Kai WANG
;
Yongan ZHOU
;
Liping SU
;
Jianrui WU
;
Jufen XIE
;
Jin ZHAO
;
Li MA
- Publication Type:Journal Article
- Keywords:
Leukemia;
lymphoblastic;
acute;
TEL-AML1 fusion gene;
Polymerase chain reaction;
In situ hybridization;
fluorescence
- From:
Journal of Leukemia & Lymphoma
2009;18(11):675-678
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect expression of TEL-AML1 fusion genes in pediatric cases with acute lymphoblastic leukemia(ALL) and discuss the role of reverse transcriptase polymerase chain reaction(RT-PCR)and fluorescence in situ hybridization(FISH) in detection of t(12 ;21) and the clinical significance. Methods TEL-AML1 fusion gene was identified in bone marrow munonuclear cells from 31 newly diagnosed childhood ALL patients by NRT-PCR, FISH and conventional cytogenetic analysis (CCA). Results TEL-AML1 fusion gene was found in 7 out of 31 cases, accounting for 22.6 % in pediatric ALL, and 7 out of 31 cases accounting for 25.9 % in B-ALL Seven cases were found with t (12;21) by FISH and NRT-PCR. The incidence of the t(12;21) was 22.6 % in newly diagnosed pediatric ALLs. Conclusion It is concluded that TEL-AML1 rearrangement is a frequent molecular abnormality in childhood ALL. t(12;21) is the most common cytogenetic translocations in Chinese pediatric ALLs, but it is always difficult to identify by routine CCA.Other molecular methods, e.g. NRT-PCR and FISH are powerful in detecting such a critical genetic translocation.
