Effect of NF-κB signaling pathway on the formation of multidrug resistance in K562/A02 cells
10.3760/cma.j.issn.1009-9921.2009.03.003
- VernacularTitle:NF-κB信号通路的激活对白血病细胞K562/A02多药耐药的影响
- Author:
Haiying SUN
;
Dezhi LI
;
Kailin XU
;
Zhenyu LI
;
Lingyu ZENG
;
Qunxian LU
- Publication Type:Journal Article
- Keywords:
K562 cell;
K562/A02;
NF-κB;
P-glycoprotein;
Polymerase chain reaction
- From:
Journal of Leukemia & Lymphoma
2009;18(3):134-136
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the different expression of NF-κB in both K562 and its multidrug resistant cell line K562/A02 and discuss the mechanism of muhidrug resistance(MDR). Methods To detect the growing feature of the cells. Flow cytometry was used to analys the difference between the distribution profile of K562/S and K562/A02 cell. MTT colorimetry was used to determine the cytotoxic effect of adramycin, and expression of mdrl gene was detected by semi-quantitative reverse transcriptase poly-merase chain reaction (RT-PCR) in K562 and K562/A02 cells. FACS was used to determine the expression and function of glycoprotein (P-gp) on the cell membrane. Western blotting was used to determine the NF-κB p65protein in nueleus. Results There was a difference between K562 and K562/A02 cells growed in a halfadherent way rather than suspending ones, there were increases in the percentage number of cells at G0/G1 and S phases(P ＜0.05). This was mirrored by a decreasing number of cells within the G2/M phase(P＜0.05). Butthere was no difference in apoptosis rate(P ＞0.05). mdr1 mRNA was detected in K562/A02 cells, in which the expression P-gp was much higher [(94.17±0.89)%:(1.41 ±O.491)%]. NF-κB p65 protein in nucleus was overexpressed in K562/A02 cells. Conclusion The activation of NF-κB signaling pathway may attribute to the formation of MDR in K562/A02 cells.
