Inactivation of DAPK1 gene by methylated oligonucleotides and its effect on the proliferation of leukemia cell line K562
10.3760/cma.j.issn.1009-9921.2011.05.005
- VernacularTitle:甲基化寡核苷酸灭活死亡相关蛋白激酶1基因对白血病K562细胞增殖的影响
- Author:
Fei ZHAN
;
Junhua LI
;
Feng CHEN
;
Ming CHEN
- Publication Type:Journal Article
- Keywords:
Leukemia;
Neoplasms,experiment;
K562 cells;
Methylated oligonucleotide;
Death associated protein kinase 1 (DAPK1);
Promoter regions (Genetics);
Cell proliferation
- From:
Journal of Leukemia & Lymphoma
2011;20(5):269-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective To inactivate Death-associated protein kinase 1 gene (DAPK1) by transfecting complementary methylated oligonucleotides and studies its effect on the proliferation of myelogenous leukemia cell line K562. Methods Methylated oligonucleotides complementary to DAPK1 gene promoter were transfected into K562 cells by Iipo2000. Methylation specific PCR (MSP) and Reverse transcription PCR (RT-PCR) were applied to detect DAPK1 gene promoter methylation status and its mRNA expressions respectively. MTT was used to detect the proliferation of K562 cells pre- and post- oligonucleotides transfection. Results DAPK1 gene promoter in non-treated and control groups were unmethylated with detectable mRNA expressions, but it became methylated with inhibited mRNA expressions after methylated oligonucleotide transfection. Proliferation in methylated oligonucleotide treatment group was significantly higher than that in non-treated and control groups. Conclusion Complementary methylated oligonucleotides could inactivate DAPK1 gene and inhibit its expression in K562 cells, which could promote its proliferation.
