The Effect of Staphylococcus Aureus on Expression of iNOS and IL-1β in THP- 1 Monocytes under High Glucose Concentration
10.3969/j.issn.0253-9896.2010.01.004
- VernacularTitle:金葡菌对高糖状态下THP-1人单核细胞表达iNOS和IL-1β的影响
- Author:
Ying CHEN
;
Dong MENG
;
Pei SUN
;
Baoli WANG
;
Weimin DENG
;
Zhi YAO
- Publication Type:Journal Article
- Keywords:
monocytes;
staphylococcus aureus;
infection;
glucose;
nitric oxide synthase;
interleukin-1beta
- From:
Tianjin Medical Journal
2010;38(1):11-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of staphylococcus aureus on expressions of inducible nitric oxide synthase (iNOS) and interleukin-1 beta (IL-1β) in THP-1 monocytes under high glucose concentrations. Methods:THP- 1 monocytes were incubated at different surroundings with 2×2 factorial design. There were 4 experimental groups in the study, which were analyzed by univariate analysis of variance. The total RNA was distilled. The expressions of iNOS and IL-1β were examined by SQ-RT-PCR. Results:The expressions of iNOS and IL-1β were affected by high glucose concentration, bacteria and both of them together in THP-1 monocytes. The expressions of iNOS and IL-1β were weakened in high glucose concentration compared with that of control(P < 0.01). The expressions of iNOS and IL-1β were weakened in the high glucose concentration and bacterial infection compared with that of the high glucose concentration only(P < 0.01). The expressions of iNOS and IL-1β increased in bacterial infection compared with that of control(P < 0.01). The expression of IL-1β increased in high glucose concentration and bacterial infection compared with that of the high glucose concentration only(P < 0.01) but the expression of iNOS was not distinct. Conclusion:When staphylococcus aureus infected monocytes, the expressions of iNOS and IL-1β were weakened in high glucose concentration, which suggested that the depressed function of immunocyte was related with high glucose concentration.
