Effect of combination with magnetic nanoparticle of Fe3O4 and adriamycin on Raji cell line
10.3760/cma.j.issn.1009-9921.2011.12.009
- VernacularTitle:磁性纳米铁联合多柔比星对Raji细胞的影响
- Author:
Jing WANG
;
Ping YANG
;
Hongmei JING
;
Xiaoyan KE
- Publication Type:Journal Article
- Keywords:
Magnetic nanoparticle of Fe3O4;
Adriamycin;
Raji cells;
p53;
NF-κB
- From:
Journal of Leukemia & Lymphoma
2011;20(12):730-733
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of combination with magnetic nanoparticle of Fe3O4and adriamycin (ADM) on Raji cell line.Methods Raji cells were cultured with Fe3O4-magnetic nanoparticle and ADM after using mechanical absortion polymerization,and the cell viability was detected by MTT and trypan-blue exclusion.The apoptosis was detected by flow cytometry,and the expression of p53 and NF-κB were measured by Western blot. Results Raji cell proliferation ratio was significantly inhibited in concentration-and time-dependent manners in both ADM and Fe3O4-MNP-ADM groups (r =0.412,P =0.027;r =0.523,P =0.014).The percentages of apoptotic cells induced by ADM and Fe3O4-MNP-ADM were 8.76 ％ vs 14.85 ％,35.08 ％ vs 44.50 ％,44.00 ％ vs 69.40 ％ in 12,24 and 48 hours,respectively (t =-9.137,-4.808,-6.337; P =0.012,0.041,0.024).The grey straps of NF-κB measured by Western blot were 4.22±0.32,3.31±0.28 in ADM and Fe3O4-MNP-ADM group (t =-54.416,P =0.035),whereas the grey straps of p53 were 1.042±0.114,1.270±0.091,respectively (t =33.963,P =0.047).Conclusion Combination therapy of Fe3O4-MNP and ADM could inhibit proliferation and induce apoptosis of Raji cells line,which may due to upregulation of p53 and down-regulation of NF-κB.
