Expression and significance of FasL and FAP-1 in acute myeloid leukemia
10.3760/cma.j.issn.1009-9921.2009.08.014
- VernacularTitle:FasL和FAP-1基因在急性髓系白血病中表达的临床意义
- Author:
Yaqing FENG
;
Zhenhua QIAO
- Publication Type:Journal Article
- Keywords:
Leukemia;
myeloid;
acute;
RT-PCR;
Fas ligant protein;
FAP-1
- From:
Journal of Leukemia & Lymphoma
2009;18(8):481-483
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of the mRNA level of Fas (CD95) ligand/FasL and Fas-associated phosphatase-1/FAP-1 in acute myeloid leukemia. Methods The expression of FasL and FAP-1 were detected in 54 patients with AML and 10 normal subjects by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). β-actin used as internal reference. The changes of FasL was observed after induction chemotherapy in 16 AML patients. The expression of Fas was detected in 54 patients with AML by flow cytometry. Results The mRNA levels of FasL and in 54 patients were remarkably higher(P <0.05) than the normal controls. The rates of first complete remission(CR) were significantly higher in FasL(+) (77.78 %) than in FasL(-) cases(16.67 %) (P<0.01). The FasL level declined in 10 patients with good response to therapy (P<0.01), and high level in 6 nonresponding patients with poor response (P>0.05). The mRNA levels of FAP-1 in 54 patients was remarkly higher than that of the normal control. 8/29 cases in Fas-positive group were positive for FAP-1 mRNA expression. 19/25 cases in the Fas-refractory group didn't express FAP-1 mRNA. Conclusion The expression of FasL was high in AML. The rates of complete remission were high in FasL positive cases. The FasL level declined in patients with good response to therapy. The expression of FAP-1was partly expressed in AML. The expression of FAP-1 was less in Fas positive group.
