Expression and clinical implication of HIF-1α mRNA in mononuclear cells of bone marrow of multiple myeloma
10.3760/cma.j.issn.1009-9921.2011.04.012
- VernacularTitle:多发性骨髓瘤骨髓单个核细胞缺氧诱导因子1α mRNA的表达及其临床意义
- Author:
Junping YAO
;
Xinghong WANG
;
Dongping HUANG
;
Guiping SU
- Publication Type:Journal Article
- Keywords:
Multiple myeloma;
RNA;
Hypoxia-inducible factor 1;
Real-time fluorescence quantitative reverse transcriptase polymerase chain reaction
- From:
Journal of Leukemia & Lymphoma
2011;20(4):229-231
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the correlation between the expression of HIF-1α in multiple myeloma (MM) and clinical indexes in order to illustrate the expression and implication of HIF-1α gene in MM. Methods RQ-PCR method was used to amplify HIF-1α mRNA of bone marrow mononuclear cells isolated from 28 cases of MM patients and the control group. β-actin was used as internal standard. HIF-1α mRNA expression was analyzed by SDS software and the ratios of HIF-1α/ β-actin were calculated. Results The relative expression level of HIF-1α mRNA in MM bone marrow mononuclear cells was 12.68 times as that in control group. HIF-1α mRNA was positively correlated with β2-MG (r =0.575, P =0.000), ESR (r =0.522,P =0.000), LDH (r=0.286, P=0.044) and CRP (r =0.356, P =0.011). There was a negative correlation between HIF-1α mRNA and Hb (r =-0.556, P =0.000). Conclusion The expression of HIF-1α mRNA was up-regulated and HIF-1α was related to a number of clinical indexes. HIF-1α may be used to estimate the progress of MM and hopeful to be a new molecular target in cancer therapy.
