Inhibition of gene p15 hypermethylation by phenylhexyl isothiocyanate in Molt-4 cells
10.3760/cma.j.issn.1009-9921.2009.02.005
- VernacularTitle:异硫氰酸苯己酯诱导Molt-4细胞p15基因去甲基化的实验研究
- Author:
Xudong MA
;
Shaohong JIANG
;
Yiqun HUANG
;
Yunlu XU
;
Ruiji ZHENG
- Publication Type:Journal Article
- Keywords:
Methylation;
Histone deacetylase;
Proteinase inhibitors;
Phenylhexyle isothiocyanate
- From:
Journal of Leukemia & Lymphoma
2009;18(2):79-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of phenylhexyle isothiocyanate (PHI) on demethylation and activation of transcription gene p15 in acute leukemia cell line Molt-4. Methods DNA sequencing and modified methylation specific PCR (MSP) were used to screen p15-M and p15-U mRNA after Moh-4 cells were treated with PHI. P15 mRNA was measured by RT-PCR. Pl5 protein was detected by Western blotting. Results Hypermethylation of gene pl5 was apparently attenuated and activation of transcription p15 gene was de novo after 5 days exposure to PHI. PHI enhanced both the expression of p15 mRNA and p15 protein in a concentration-dependent manner. The ratio of the gray scale of p15 mRNA strap was 0.17±0.12 in control, 0.29±0.14 in PHI 10 μmol/L, 0.55±0.07 in PHI 20 μmol/L, 0.93±0.13 in PHI 40 μmol/L. Conclusion PHI could active demethylation and transcription of gene p15.
