Analysis of point mutation and deletion of intron 14 in the juxtamembrane domain of Flt3 gene in leukemia
10.3760/cma.j.issn.1009-9921.2011.07.006
- VernacularTitle:白血病Flt3基因编码的近膜区点突变的检测和内含子14缺失的分析
- Author:
Min ZHAO
;
Xiaohua WANG
;
Tiechen LI
;
Yuehua ZHAO
- Publication Type:Journal Article
- Keywords:
Flt3;
Leukemia;
Juxtamembrane domain;
Point mutation;
Intron deletion
- From:
Journal of Leukemia & Lymphoma
2011;20(7):401-403
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the pathogenesis of leukemia and Flt3 gene by detecting the point mutations in juxtamembrane domain coding by Flt3. Methods The exons 14, 15 of Flt3 gene were detected using PCR, PCR/SSCP and gene sequence in 60 leukemia patients with peripheral blood and bone marrow samples. Results We found Flt3-L576P point mutation in 3 cases in juxtamembrane domain in 60 patients, including 1 patient had a deletion of intron 14. Conclusion Flt3-L576 was a new point mutation site, which way be associated with the pathogenesis and development of leukemia.
