Expression and function of chemokine CCL2 in the bone marrow cells of patients with acute lymphoblastic leukemia
10.3760/cma.j.issn.1009-9921.2012.02.014
- VernacularTitle:急性淋巴细胞白血病骨髓细胞单核细胞趋化蛋白-1的表达及其意义
- Author:
Xiaotao WANG
;
Yang QIN
;
Ailin TANG
;
Yuwei NIE
;
Jian LIU
- Publication Type:Journal Article
- Keywords:
Leukemia,biphenotypic,acute;
Bone marrow mesenchyaml cells;
Bone marrow mononuclear cells;
Monocyte chemoattractant proteins
- From:
Journal of Leukemia & Lymphoma
2012;21(2):111-114
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression of CCL2 and its effects on the proliferation and adhesiveness on leukemia cells in patients with acute lymphoblastic leukemia(ALL). MethodsThe bone marrow mesenchymal cells (BMMSC) and bone marrow mononuclear cells (BMMNC) of 30 ALL patients and 30 healthy controls were studied, and CCL2 level was evaluated by ELISA. CCL2 gene mRNA level in ALL was determined by RT-PCR. The cell proliferation and adhesiveness were detected by MTT assays. The cell counts were measured by flow cytometry. ResultsThe BM plasma levels of CCL2 in patients at diagnosis were significantly higher than that in healthy controls [(780.12±129.61) pg/ml vs (120.49±25.21) pg/ml,t =4.96, P =0.00]. Supernatant levels of CCL2 in BMMSC were significantly higher than that of BMMNC [(572.38±35.39) pg/ml vs (193.85±15.45) pg/ml,t =5.37,P =0.00]in vitro.CCL2 cannot induce leukemia proliferation alone,but could induce leukemia proliferation in BMMNC and BMMSC co-culture in a dose- and time-dependent manner.CCL2 could increase the leukemia adhesive to the BMMSC compared with control (r =0.824,P =0.02).ConclusionPatients with B type ALL had higher levels of CCL2 which was secreted by BMMSC. The leukemia could induce the BMMSC to secrete CCL2. CCL2 could promote the survival and proliferation of leukemia in the presence of BMMSC and BMMNC, and enhance ALL cells adhesion toBMMSC in a dose-dependent manner.
