Expression of Rho GTPase family member RhoA and CDC42 protein in patients with acute leukemia
10.3760/cma.j.issn.1009-9921.2010.06.003
- VernacularTitle:RhoA和CDC42在急性白血病的表达异常及其意义
- Author:
Jiying WANG
;
Jianwei ZHANG
;
Yan LI
;
Kejing TANG
;
Yanzhong WANG
;
Min WANG
;
Haiyan XING
;
Zheng TIAN
;
Qing RAO
- Publication Type:Journal Article
- Keywords:
Leukemia,acute;
RhoA;
CDC42 GTP-binding protein;
Western blotting
- From:
Journal of Leukemia & Lymphoma
2010;19(6):327-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the proteins levels of RhoA and CDC42 in bone marrow mononucleated cells (BMMC) of patients with primary acute leukemia,and further determine the role of abnormal interactions between hematopoietic progenitor and bone marrow microenvironment on abnormal behaviors of leukemia cells. Methods BMMC samples were separated from 54 primary acute leukemia patients and 22 normal donors and the cell lysis samples were prepared. RhoA and CDC42 proteins were determined by Western blotting. Independent pair T test was conducted to evaluate whether the differences in RhoA and CDC42 expression were statistically significant between leukemia patients and normal donors. Spearman was applied in analyzing the correlation between expression of RhoA and CDC42 proteins and clinical characters of patients. Results RhoA and CDC42 proteins level of primary acute leukemia patients was significantly higher than that of normal samples. Especially, patients with M2,M3 and M5 subtypes exhibited significant higher RhoA proteins levels and M3 subtype exhibited significant higher CDC42 protein levels. Conclusion RhoA and CDC42 protein levels of primary acute leukemia patients are significantly higher than that of normal donors. This result suggests that RhoA and CDC42 associated efficient migration of leukemia cells could be implicated in abnormal interaction of leukemic cell with bone marrow microenvironment.
