In vivo MR tracking of transplanted bone marrow stromal cells labeled with Resovist in rat models with Parkinson disease
10.3321/j.issn:1003-3289.2009.12.007
- VernacularTitle:帕金森病大鼠模型定向移植Resovist标记骨髓基质干细胞的活体MR示踪
- Author:
Jing GUO
;
Lan WANG
;
Rongjun ZHANG
;
Weifeng LUO
;
Zhigang GONG
;
Jing SUN
;
Han XU
;
Junkang SHEN
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells;
Parkinson disease;
Resovist;
Magnetic resonance imaging
- From:
Chinese Journal of Medical Imaging Technology
2009;25(12):2170-2173
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the migration ability of different quantity of BMSCs labled with Resovist after transplanted in rat models with Parkinson disease (PD) with 1.5T MR scanner and micro-47 coil, in order to determine the optimal transplanted dosage of BMSCs and observation time in vivo. Methods Forty PD rats were randomly assigned to 5 groups (each n=8) including 1×10~5 BMSCs group, 1.5×10~5 BMSCs group, 2×10~5 BMSCs group, 2.5×10~5 BMSCs group and control group. FFE-T2WI were obtained immediately and 1 week, 4 weeks, 8 weeks after transplantation to measure and compare the volume of hypointense areas of different groups in different time with 1.5T and 47 mm inner diameter micro-coil. At the meantime, rotational behavior was assessed in each group. After MR scanning, the rats were executed and prepared for immunohistochemistry staining at 12 weeks after transplantation. Results Only the extent of the dark region became wider 4 weeks after transplantation in 2×10~5 BMSCs group (P=0.005). The therapeutic efficacy in 2×10~5 BMSCs group was the best confirmed by behaviour studies (P=0.02). Conclusion For the treatment of PD and evaluation of the migratory ability of BMSCs, the optimal transplanted dosage of BMSCs is 2×10~5, while the best observation time is 4 weeks after transplantation.
