Expression of Osteoclastogenesis-related Genes in Rheumatoid Arthritis Synovial Macrophages.
- Author:
Jong Dae JI
1
;
Tae Hwan KIM
;
Bitnara LEE
;
Sung Jae CHOI
;
Young Ho LEE
;
Gwan Gyu SONG
Author Information
- Publication Type:Original Article
- Keywords: Rheumatoid arthritis; Synovial osteoclast precursors; Osteoclastogenesis
- MeSH: Arthritis, Rheumatoid; Genes, vif; Macrophages; Microarray Analysis; Oligonucleotide Array Sequence Analysis; Osteoclasts; RNA, Messenger; Synovial Fluid
- From:Journal of Rheumatic Diseases 2011;18(1):11-18
- CountryRepublic of Korea
- Language:Korean
- Abstract: OBJECTIVE: To examine the mechanism for the inhibited differentiation of osteoclasts in rheumatoid arthritis synovial CD14+ osteoclast precursors, the different expressions of the osteoclastogenesis-related genes in rheumatoid arthritis (RA) synovial fluid CD14+ osteoclast precursors were compared with those of normal peripheral blood (PB) CD14+ osteoclast precursors. METHODS: The expression of osteoclastogenesis-related genes were examined using a gene expression oligonucleotide microarray. To validate the results of the microarray analysis, the mRNA expressions of osteoclastogenesis-related genes were measured by real-time PCR. RESULTS: Comparative analysis of the mRNA profiles showed significantly different expression of osteoclastogenesis- related genes, such as MafB, Id3 and LILRB4, in the RA synovial CD14+ osteoclast precursors, compared to that of normal PB CD14+ osteoclast precursors. CONCLUSION: The expression of the osteoclastogenesis-related genes in RA synovial CD14+ osteoclast precursors is different from that of the normal PB CD14+ osteoclast precursors. These results suggest that the different expression of osteoclastogenesis-related genes might be involved in the altered osteoclastogenesis in RA synovial osteoclast precursors.
