Establishment of DsRed-labled leukemia mouse model
10.3760/cma.j.issn.1009-9921.2010.09.011
- VernacularTitle:红色荧光蛋白标记的小鼠白血病模型的建立
- Author:
Chong CHEN
;
Yanjie LI
;
Jiang CAO
;
Dongyang WANG
;
Lingyu ZENG
;
Xiuying PAN
;
Kailin XU
- Publication Type:Journal Article
- Keywords:
Fluorescent antibody technique;
Leukemia,exprimental
- From:
Journal of Leukemia & Lymphoma
2010;19(9):548-551
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to establish the leukemia mouse model by using EL4/DsRed cell line expressing red fluorescent protein (DsRed) and to evaluate the model. Methods After total body irradiation with X-ray of 7.0 Gy, C57BL/6 mice were inoculated 5×106 bone marrow cells mixed different numbers of EL4/DsRed cells via tail vein. The model was evaluated by flow cytometry (FCM), reverse transcriptase-polymerase chain reaction (RT-PCR), and histopathology. Results The incidence of leukemia was 100 %. The presence of EL4/DsRed cells was found in liver, spleen, bone marrow and peripheral blood of recipients by FCM two weeks after transplantation. Pathological section revealed that all recipients had several organs infiltration apparently. With the increase in the number of inoculated tumor cells, the survival time of recipients was reduced and the infiltration of leukemia cells in organs was more serious. Conclusion Mouse leukemia model was successfully established when C57BL/6 mouse was intravenously transplanted with ≥5×102 EL4/DsRed cells. The model could be employed usefully in the future research such as the pathogenesis of leukemia and minimal residual disease (MRD).
