Immobilized enzyme reactors in HPLC and its application in inhibitor screening: A review
10.1016/j.jpha.2011.12.002
- Author:
Simeng FANG
;
Haina WANG
;
Zhongxi ZHAO
;
Weihong WANG
- Publication Type:Journal Article
- Keywords:
Immobilized enzyme reactors (IMERs);
Molecular bio-chromatography;
Screening enzyme inhibitors
- From:
Journal of Pharmaceutical Analysis
2012;02(2):83-89
- CountryChina
- Language:Chinese
-
Abstract:
This paper sets out to summarize the literatures based on immobilized enzyme biochromatography and its application in inhibitors screening in the last decade.In order to screen enzyme inhibitors from a mass of compounds in preliminary screening,multi-pore materials with good biocompatibility are used for the supports of immobilizing enzymes,and then the immobilized enzyme reactor applied as the inmobilized enzyme stationary phase in HPLC.Therefore,a technology platform of high throughput screening is gradually established to screen the enzyme inhibitors as new anti-tumor drugs.Here,we briefly summarize the selective methods of supports,immobilization techniques,co-immobilized enzymes system and the screening model.
