Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation
10.1016/j.jpha.2011.10.002
- Author:
Rajeev JAIN
;
Ramkishor SHARMA
- Publication Type:Journal Article
- Keywords:
Voltammctry;
Adefovir;
Solid-phase extraction;
Protein-precipitation;
Human plasma;
Pharmaceutical formulation
- From:
Journal of Pharmaceutical Analysis
2012;02(2):98-104
- CountryChina
- Language:Chinese
-
Abstract:
Electrochemical reduction behavior of Adefovir was studied using Hanging Mercury Drop Electrode (HMDE) in Britton-Robinson (BR) buffer solution.Voltammetric study showed one well-defined reduction peak in the potential range -1.2 to -1.4 V (vs.Ag/AgCl) due to reduction of C=N bond of the imidazole ring.Solid-phase extraction and protein-precipitation techniques were employed for extraction of Adefovir from human plasma.The proposed method allows quantification of Adefovir in human plasma over the concentration range 0.50-5.00 μg/mL with the detection limit 0.17 μg/mL,whereas in pharmaceutical formulation 0.25-2.25 μg/mL with the detection limit 0.08 μg/mL.
