Effect of stromal cell-derived factor-1 on the adherence function of mononuclear cells
10.3760/cma.j.issn.1009-9921.2008.03.005
- VernacularTitle:基质细胞衍生因子-1对脐血单个核细胞黏附作用的影响
- Author:
Ruilan YUAN
;
Yanping MA
- Publication Type:Journal Article
- Keywords:
Fetal blood;
Adherence;
Stromal cell-derived factor-1;
Very late antigen-4
- From:
Journal of Leukemia & Lymphoma
2008;17(3):175-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of stromal cell-derived factor-1 (SDF-1) on the adherence of mononuclear cells (MNC). Methods Mononuclear cells were isolated from cord blood and the adherent rate was assayed by using MTT with fibronectin coated in different concentration of SDF-1. Then the expression of adherent molecule VLA-4 by FACS as well as the adherent rate when MNC were cultured in vitro. Results The adherent rate of fresh cord blood MNC was (110.2±2.10) %, (128.3±2.09) %, (141.6±2.62) %, (160.8±3.84) %, (162.3±3.60) %, (165.4±2.73) %, (165.1±2.33) %. When the mass concentration of SDF-1 was 50, 100, 150, 200, 250 ng/ml. When MNC were cultured in vitro,the expression of VLA-4 was 26.37te was (160.2±6.38) %, (194.6±6.09) %, (147.5±6.86) %, (144.7±4.96) %, (118.1±3.64) %. The correlation coefficient between the adherent rate and the expression of VLA-4 is 0.826. Conclusion The adherent rate of fresh cord blood MNC increases along with the concentration of SDF-1, however, it tends to be stable when the concentration of SDF-1 reaches 100 ng/ml. When MNC were cultured in vitro with hemopoietic growth factors, the expression of VLA-4 and the adherent rate of MNC increased in the initial stage, however, the expression of VLA-4 and the adherent rate of MNC both decreased gradually along with time extending. There is positive correlation between the adherent rate of MNC and the expression of adherent molecule VLA-4.
