The expert system of genotype discrimination for D5S818 locus based on near-infrared spectroscopy-principal discriminant variate
10.1016/j.jpha.2011.10.007
- Author:
Zaizhen WU
;
Jianhua TANG
;
Bin ZHANG
;
Liping GUO
;
Hongping XIE
;
Bingren GU
- Publication Type:Journal Article
- Keywords:
Short tandem repeat;
Near-infrared spectroscopy;
Principal discriminant variate;
Genotyping-tree structure;
Expert system
- From:
Journal of Pharmaceutical Analysis
2012;02(2):117-122
- CountryChina
- Language:Chinese
-
Abstract:
This paper studied the expert system of genotype discrimination for the STR locus D5S818 based on near-infrared spectroscopy-principal discriminant variate (PDV).Six genotypes,i.e.genotypes 10-10,10-11,11-11,11-12,11-13 and 13-13,were selected as research subjects.Based on the optimum polymerase chain reaction (PCR) conditions,about 54 measuring samples for each genotype were obtained; these samples were tested by near-infrared spectroscopy directly.With differences between homozygote genotypes and heterozygote ones,and differences of the total number of core repeat units between the six genotypes,two types of genotyping-tree structure were constructed and their respective PDV models were studied using the near-infrared spectra of the samples as recognition variables.Finally,based on the classification ability of these two genotyping-tree structures,an optimum expert system of genotype discrimination was built using the PDV models.The result demonstrated that the built expert system had good discriminbility and robustness; without any preprocessing for PCR products,the six genotypes studied could be discriminated rapidly and correctly.It provided a methodological support for establishing an expert system of genotype discrimination for all genotypes of locus D5S818 and other STR loci.
