Demethylation stduy of decitabine in acute leukemia patients with Apaf-1 gene methylated

VernacularTitle:地西他滨对急性白血病患者甲基化凋亡蛋白酶活化因子1基因的去甲基化作用
Author: Jun GUO ; Enqin YANG ; Hui YANG
Publication Type:Journal Article
Keywords: Leukemia,acute; Apoptotic protease-activating factor 1; Demethylation; Decitabine
From: Journal of Leukemia & Lymphoma 2013;22(9):538-541
CountryChina
Language:Chinese
Abstract: Objective To investigate the effectiveness of decitabine demethylation in treatment of acute leukemia.Methods Methylation specific PCR (MSP) was used to detected the methylation status of Apaf-1 gene promoter.10 cases entering the group.MSP was used to detected the 10 cases methylation status of Apaf-1 promoter between pre-and post-treatment of dicitabine.RT-PCR method used was to detect the differential expression levels of Apaf-1 mRNA in acute leukemia bone marrow mononuclear cell between preand post-treatment of decitabine.Results In post-treatment of decitabine,6 cases Apaf-1 gene promoter was demethylated.The loss expression of Apaf-1 mRNA re-expressed in 4 cases.6 cases Apaf-1 mRNA still express deletion.6 cases patients have Apaf-1 mRNA exprssion deletion,However,4 cases Apaf-1 gene was demethylated,2 cases methylated in post-treatment,maybe related to allele deletion or allelic varriants.Conclusion Post treatment of decitabine.Apaf-1 gene promotor was demethylated and repress the expression of Apaf-1 mRNA,play a key role in apoptosis maybe a new method for treatment of acute leukemia.