Effects of bone marrow stromal cells and antibody against late antigen-4 combined with Ara-C on proliferation and apoptosis of HL-60 cells
10.3760/cma.j.issn.1009-9921.2013.09.008
- VernacularTitle:骨髓基质细胞及抗晚期抗原4抗体联合阿糖胞苷对急性髓系白血病HL-60细胞增殖和凋亡的影响
- Author:
Yating ZHANG
;
Jianpei FANG
;
Wenjun WENG
;
Lyuhong XU
;
Hao ZHUANG
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells;
Leukemia;
Antibody against late antigen-4;
Cell proliferation;
Apoptosis
- From:
Journal of Leukemia & Lymphoma
2013;22(9):535-537
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the bone marrow stromal cells,anti-late antigen-4 (VLA-4) antibody (aVLA-4),cytarabine (Ara-C) on the proliferation and apoptosis of leukemia HL-60 cells.Methods The experiment was divided into five groups:HL-60 cells were cultured alone (control group),HL-60 cells and stromal cells group (stromal cells group),HL-60 cells + stromal cells + aVLA-4 (antibody group),HL-60 cells + stromal cells + Ara-C group (drug group),HL-60 cells + stromal cells + aVLA-4 + Ara-C group (antibody +drug group).Cell proliferation or inhibition rate was detected by CCK-8 method,the HL-60 cells apoptosis was detected by flow cytometry.The expression of anti-apoptotic gene bcl-2 in HL-60 cells was determined by Western blot.Results After 24 h and 48 h,treatment,the number of the stromal cells group HL-60 cells were higher than that of the control group with significant difference cultured [(7.2±0.3)×1O5/ml vs (5.3±0.4)×105/ml,(8.4±0.2)×105/ml vs (6.8±0.3)×105/m1,P ＜ 0.001],while the HL-60 cell proliferation inhibition rate [(24.3±2.1) ％,(37.0±2.6) ％,(65.6±3.8) ％] and apoptosis rate [(5.7±0.6) ％,(8.0±0.5) ％,(10.4±0.9) ％,(16.5±0.7) ％] of antibody group,drug group,antibody + drug group were higher than the control group with a difference of statistically significant (P ＜ 0.05),and the increase of antibody + drug group was most obvious.With the decreasing of the bcl-2 protein expression,which was most the decrease of antibody + drug group was most obvious.Conclusion Bone marrow stromal cells can stimulate the proliferation of leukemia cells,aVLA-4 interference the interaction between stromal cells and leukemia cells can enhance the chemosensitivity of leukemia cells to Ara-C.
