Construction and identification of eukaryotic expressing vector for multiple myeloma MUC1-2VNTR

VernacularTitle:多发性骨髓瘤MUC1-2VNTR真核表达载体构建及鉴定
Author: Yunjiao LUO ; Kun LIU ; Yuebo LIU ; Hong YANG ; Jin YAO ; Liang SHAO ; You ZHANG
Publication Type:Journal Article
Keywords: Multiple myeloma; MUC1 mucin; pcDNA3.1; Vector construction
From: Journal of Leukemia & Lymphoma 2008;17(5):334-336
CountryChina
Language:Chinese
Abstract: Objective To construct multiple myeloma mucin MUC1-2VNTR gene eukaryotic expressing vector,which provided the basic material for further study of multiple myeloma DNA vaccine.Methods MUC1-2VNTR coding gene as target gene,and a KOZAK sequence was inserted before it.Hind Ⅲ and Xba Ⅰ restriction enzyme site were inserted on both ends.Then the whole sequence was synthesized and cloned into pcDNA3.1/myc-his B vector,and the recombinant vector was identified by restriction enzyme digestion and DNA sequencing.Results Synthesized MUC1-2VNTR gene was 140 bp.Restriction enzyme digestion and DNA sequencing confirmed pcDNA3.1/MUC1-2VNTR/myc-his B including the whole exact translation frame region and MUC1-2VNTR gene.Condnsion The pcDNA3.1/MUC1-2VNTR/myc-his B has been successfully constructed,which provides the basic material for further studies of MUC1 mucin function and multiple myloma DNA vaccine.