Highly sensitive chemiluminescence technology for protein detection using aptamer-based rolling circle amplification platform
10.1016/j.jpha.2011.06.002
- Author:
Zhijuan CAO
;
Qianwen PENG
;
Xue QIU
;
Caiyun LIU
;
Jianzhong LU
- Publication Type:Journal Article
- Keywords:
Protein;
Aptamer;
Chemiluminescence;
Rolling circle amplification
- From:
Journal of Pharmaceutical Analysis
2011;01(3):159-165
- CountryChina
- Language:Chinese
-
Abstract:
A robust,selective and highly sensitive chemiluminescent (CL) platform for protein assay was presented in this paper.This novel CL approach utilized rolling circle amplification (RCA) as a signal enhancement technique and the 96-well plate as the immobilization and separation carrier.Typically,the antibody immobilized on the surface of 96-well plate was sandwiched with the protein target and the aptamer-primer sequence.This aptamer-primer sequence was then employed as the primer of RCA.Based on this design,a number of the biotinylated probes and streptavidin-horseradish peroxidase (SA-HRP) were captured on the plate,and the CL signal was amplified.In summary,our results demonstrated a robust biosensor with a detection limit of 10 fM that is easy to be established and utilized,and devoid of light source.Therefore,this new technique will broaden the perspective for future development of DNA-based biosensors for the detection of other protein biomarkers related to clinical diseases,by taking advantages of high sensitivity and selectivity.
