Research on Polyamidoamine Dendrimers Mediated Foreign Gene for Xenotransplantation Transfected Pig Sperm Cells
10.3969/j.issn.0253-9896.2010.02.013
- VernacularTitle:PAMAM-D对异种移植用外源基因转染猪精子细胞介导作用的研究
- Author:
Huixiang YANG
;
Baomin QIAO
;
Guangyou WANG
;
Shengzhi LI
;
Yue ZHANG
;
Changping LI
;
Yong XU
;
Tengxiang MA
- Publication Type:Journal Article
- Keywords:
spermatozoa;
transplantation,heterologous;
transfection;
genes;
swine
- From:
Tianjin Medical Journal
2010;38(2):121-123
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of the new nanomaterials,polyamidoamine dendrimers PAMAM-D,mediated foreign gene human decay accelerating factor(hDAF) for xenotransplantation transfected pig sperm cells.Methods:PAMAM-D/hDAF cDNA compounds were made.The compounds were divided into 0.2 μg,0.4μg,0.6μg,0.8 μg and 1.0μg groups (each group adding corresponding dose of PAMAM-D in accordance with the N/P ratio 10:1,20:1,40:1),then were digested by restriction enzymes.The compounds were incubated with washed pig sperm cells.Then the transfection efficiency was detected by in situ hybridization in the different groups.Results:The PAMAM-D molecule can prevent electrophoretic migration of DNA in the compound.After digested the compounds by restriction enzymes,DNA can not be degraded.The transfection efficiency was different in different groups.Among the total,the efficiency was higher in both groups of 0.4 μg and 0.6μg than that of others.The top was the group of 0.4 μg linear plasmid plus PAMAM-D when the N/P ratio was 20:1(47.5%±O.2%,167% vs control group).Conclusion:PAMAM-D can improve the efficiency of exogenous gene transfeeted pig sperm cells,which can reinforce the stable binding of exogenous DNA to sperm cells.
