Interleukin-6 Inhibits the Expression of Apolipoprotein M in HepG2 Cells
10.3969/j.issn.0253-9896.2010.03.019
- VernacularTitle:白细胞介素-6对肝细胞载脂蛋白M表达的影响
- Author:
Li LI
;
Gang GUO
;
Yunzhao TANG
;
Fujun SUN
;
Daiqing LI
- Publication Type:Journal Article
- Keywords:
apolipoproteins;
interleukin-6;
hepatocytes;
polymerase chain reaction;
cells,cultured
- From:
Tianjin Medical Journal
2010;38(3):216-218
- CountryChina
- Language:Chinese
-
Abstract:
Obiective:To investigate the effect of interleukin-6(IL-6)on apolipoprotein(apo)M expression in a human hepatoblastoma cell line(HepG2).Methods:HepG2 cells were cultured and incubated with different concentrations of IL-6 (0,1.25,2.5,5,10,20,40 and 80 μg/L)for 24 hours.After the incubations,total RNAs were extracted and applied to reverse transcript PCR and real-time quantitative PCR to detect the expression levels of apoM and apoA-I.The effect of IL-1α on apoM expression was also determined.Results:IL-6 significantly inhibited the expression of apoM(F=10.778,P ＜ 0.01).Whereas IL-6 did not influence the expression of apoA-I(F=2.004,P ＞ 0.05).IL-1α(0,1.25,2.5,5,10,20,40 and 80 μg/L)did not decrease the expression of apoM(F=2.038,P ＞ 0.05).Cooclusion:IL-6 inhibits apoM mRNA transcription,which may contribute to the pathogenesis of abnormal lipid metabolism and macrovascular complications in type 2 diabetes.
