The effects of Newcastle disease virus on human leukemia in vitro

VernacularTitle:新城疫病毒体外抗白血病作用的实验研究
Author: Yajun WANG ; Jianbai ZHANG ; Xiaohui LI ; Chun SONG
Publication Type:Journal Article
Keywords: Newcastle disease virus; Leukemic,monocytic,acute
From: Journal of Leukemia & Lymphoma 2010;19(9):545-547
CountryChina
Language:Chinese
Abstract: Objective To study the effects of Newcastle disease virus (NDV) on human leukemia in vitro. Methods The density and morphologic change of cells infected by NDV was observed. MTT assay was used to investigate the effect of NDV on the proliferation of the leukemia cells. Results The cells of the control were all regular and coarctate by inverted microscope. The effect of NDV were conspicuous in that the cells were irregular, sparsate, aggregate and fused in the experimental groups. MTT assay showed that the proliferation of SHI-1 cells were significantly inhibited by NDV with different concentration (P ＜0.01). The results also demonstrated that the difference within the concentration and the time were both statistically significant (P＜0.01). The inhibition of NDV was further confirmed in a time-concentration-dependent manner.Conclusion Newcastle disease virus can inhibit human acute monocytic leukemic cell proliferation in vitro.The safety of NDV is reliable.