Absence of Fanconi anemia proteins in acute myeloid leukemia cell lines THP-1 and M-07e

VernacularTitle:急性髓系白血病细胞株THP-1和M-07e中范可尼贫血相关蛋白表达缺失
Author: Hui XIAO ; Kejian ZHANG ; Bing XIA
Publication Type:Journal Article
Keywords: Leukaemia,myeloid,acute; Fanconi anemia complementation group proteins; Genomic instability
From: Journal of Leukemia & Lymphoma 2008;17(4):248-251
CountryChina
Language:Chinese
Abstract: Objective To find the possible relationship between defects in the FA/BRCA pathway of genomic maintenance and potential pathogenesis of acute myeloid leukaemia.Methods Twenty-five AML cell lines derived from diverse subtypes of AML were screened to investigate for possible defects in FA,BRCA patllway.Results The absence of FANCN protein in two cell lines,THP-1 and M-07e were observed,which was correlated with the results of MMC-induced G2 arrest,growth inhibition and chromosomal breakage test in both cell lines.But no gene aberrance in these two cell lines by MLPA test and DNA sequencing were found.Conclusion There is a possible relationship between defects in the FA/BRCA Dathway and potentialpathogenesis of acute myeloid leukaemia.A disturbance of theFA pathwaymay represent an early event in the development of this type of leukaemia.