Biotin-streptavidin system-mediated folate receptor-targeted quantum dot in vitro imaging of ovarian cancer SKOV3 cells
10.3969/j.issn.1000-8179.20140842
- VernacularTitle:生物素-链霉亲和素介导受体靶向卵巢癌SKOV3细胞量子点体外成像的研究
- Author:
Liju NIE
;
Hengyi XU
;
Chenlian YE
;
Xiaolin HUANG
;
Wenting LIU
;
Fen FU
- Publication Type:Journal Article
- Keywords:
biotin-streptavidin;
folic acid;
quantum dots;
ovarian cancer cell;
imaging in vitro
- From:
Chinese Journal of Clinical Oncology
2014;(19):1254-1258
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a biotin-streptavidin system (BAS)-mediated folate receptor (FR)-targeted quantum dot (QD) fluorescent probe and preliminarily validate the targeting ability and signal amplification effect of the probe. Methods: Streptavidin (SA) was covalently coupled with QD through the active ester method;the physical characteristics of the prepared QD-SA were veri-fied. Biotinylated folate was synthesized through the carrier bovine serum albumin using the same method and then reacted with QD-SA to form the special probe. The probe was used to identify SKOV3 cells and FR-negative A549 cells to verify its targeting speci-ficity. QD-SA was used as the contrast. SKOV3 cells were imaged using the BAS-mediated FR-targeted QD probe with a biotinylated folate incubation time of 1 or 4 h. Various reaction times were also tested between the probe and the QD-FA that was formed without BAS mediation. Results:The BAS-mediated FR-targeted QD probe specifically recognized FR-positive SKOV3 cells. The probe ob-tained higher fluorescent intensity after 4 h than after 1 h of biotinylated folate incubation. The BAS-mediated FR-targeted QD probe al-so had a stronger fluorescent signal than the QD-FA probe. Conclusion:The proposed probe presents a great potential in the early diag-nosis of ovarian cancer because of its high specificity and sensitivity.
