MRI of the central nervous system in rats of multiple sclerosis with clinical whole body MR scanner
10.3321/j.issn:1003-3289.2009.10.001
- VernacularTitle:临床型MR观察多发性硬化模型大鼠中枢神经系统
- Author:
Haiqin ZHANG
;
Kuncheng LI
;
Chunshui YU
;
Wen QIN
;
Jia MA
- Publication Type:Journal Article
- Keywords:
Magnetic resonance imaging;
Rats;
Multiple sclerosis;
Experimental autoimmune encephalomyelitis
- From:
Chinese Journal of Medical Imaging Technology
2009;25(10):1729-1732
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of using clinical whole body MR scanners to investigate the intravital visibility of central nervous system (CNS) lesions in rats of multiple sclerosis (MS). Methods Ten Lewis rats were injected with the peptide 35-55 of myelin oligodendrocyte glycoprotein to make the model of MS. On a Siemens Sonata 1.5T MR scanner equipped with a flexible surface coil, rats brain and spinal cord were examined using T2-weighted and T1-weighted imaging with slice thickness of 1-2 mm. On a Siemens Trio Tim 3.0T MR-scanner equipped with a quadrature wrist coil, rats were examined using T2WI, T1WI and Gd-DTPA enhanced T1WI 3-dimensional imaging with voxel size up to 0.06-0.08 mm~3. Rat brain and spinal cord images in multiple orientations were reconstituted with special software in workstation. Results T2WI and T1WI of the lesions in MS rat brain with high spatial and contrast resolution could be obtained with clinical 3.0T MR scanner, though the image resolution of spinal cord was relatively low. The resolution of 1.5T MR was lower than that of 3.0T. Plaques in CNS of MS rats presented as hyperintense areas on T2WI and hypointense areas on T1WI. Contrast enhancement was observed as hyperintense on T1WI. Conclusion High quality images of CNS lesions canbe obtained with clinical 3.0T MR-scanner in MS rat, which offers a noninvasive access for studying CNS diseases in the rats.
