Synthesis of a new chitosan derivative and assay of Escherichia coli adsorption
- Author:
Bingbing SHANG
;
Jun SHA
;
Yang LIU
;
Qin TU
;
Manlin LI
;
Jinyi WANG
- Publication Type:Journal Article
- Keywords:
chitosan derivative;
synthesis;
bacterial adhesion;
biofilm
- From:
Journal of Pharmaceutical Analysis
2011;01(1):39-45
- CountryChina
- Language:Chinese
-
Abstract:
A new chitosan derivative is prepared using chitosan.Ethyl chlorocarbonate was first introduced to the hydroxyl group of phthaloylchitosan through a nucleophilic reaction.Hydrazine was then added to recover the amino groups of chitosan and promote cross-linking.The structure of this new chitosan derivative was characterized by Fourier transform infrared (FT-IR) and proton nuclear magnetic resonance (1H NMR) spectroscopy,and its physical properties were determined by X-ray diffraction (XRD),differential scanning calorimetry (DSC),and thermogravimetric analysis (TGA).The thermal and chemical stabilities of the new derivative were improved compared with those of native chitosan.Assay of Escherichia coli adhesion on a film based on this chitosan derivative showed good adsorption and biofilm formation.
