Role of the JAK2-STAT3 Pathway Mediates the Cardioprotection of Ischemic Postconditioning
10.3969/j.issn.0253-9896.2010.01.014
- VernacularTitle:JAK2-STAT3通路介导缺血后处理心肌保护作用的初步研究
- Author:
Dachuan XIA
;
Yikui TIAN
;
Zhihao WU
;
Minxin WEI
- Publication Type:Journal Article
- Keywords:
myocardial reperfusion injury;
myocardial infarction;
apoptosis;
STAT3 transcription factor;
rats;
Wistar ischemic postconditioning
- From:
Tianjin Medical Journal
2010;38(1):43-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of ischemic postconditioning on myocardial infarction and myocardial apoptosis in ischemia-reperfusion injury of rats, and the protective mechanisms thereof. Methods:Thirty-six healthy male Wistar rats(230-280 g) were randomly divided into three groups, ischemia-reperfusion group(Group I-R), ischemia 30 min and reperfusion 120 min without additional intervention; myocardial ischemic postconditioning group(Group Post), after 30 min ischemia, the rats were comprised 3 cycles of 10 seconds reperfusion followed by 10 seconds ischemia, and then the rats were reperfused 120 min; myocardial ischemic postconditioning+AG490(JAK2-STAT3 pathway inhibitor) group(Group Post+AG490), rats were treated with AG490 5 minutes before reperfusion, followed by myocardial ischemic postconditioning and 120 min reperfusion. The myocardial infarct size was measured by TTC staining. Apoptotic index of cardiomyocyte was detected by TUNEL. Results: Myocardial infarct size and myocardium apoptotic index were significantly reduced in Group Post compared to those in Group I-R(P ＜ 0.05). AG490 inhibited cardioprotective effect of ischemic postconditioning. Conclusion: Ischemic postconditioning provides potent cardioprotective effect. JAK2-STAT3 pathway mediates the cardioprotective effects of ischemic postconditioning.
