In vivo MR tracking of Feridex labeled bone marrow mononuclear cells in canine myocardial infarction
10.3321/j.issn:1003-3289.2009.12.008
- VernacularTitle:菲立磁标记骨髓单个核细胞移植犬心肌梗死区的活体MR示踪
- Author:
Xuefeng DENG
;
Changqing GAO
;
Liuquan CHENG
;
Libing LI
;
Haiyue JU
;
Naixiang HUANG
- Publication Type:Journal Article
- Keywords:
Magnetic resonance imaging;
Superparamagnetic iron oxides;
Feridex;
Bone marrow mononuclear cells;
Myocardial infarction
- From:
Chinese Journal of Medical Imaging Technology
2009;25(12):2174-2177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To track the magnetically labeled bone marrow mononuclear stem cells (BM-MNCs) in canine myocardial infarction (MI) model with MR. Methods BM-MNCs were labeled with Feridex effectively in vitro and then injected intramyocardially in 8 MI model dogs. Serial MR was performed with 1.5T MR scanner to show the location of the labeled cells compared with histology. Results The injection sites of labeled BM-MNCs could be located on the 1st and 2nd week, but disappeared on the 4th week. Corresponding to these sites, Prussian blue staining consistently showed that large clusters of cells were labeled by dense intracellular iron at the scar tissue. Conclusion Feridex labeling BM-MNCs enables ready detection in the beating heart on a conventional MR scanner after transplantation into canine infarcted myocardium.
