Regulating Effects of Different Moxibustion Therapies on Bax and Bcl-2 in Rats with Ulcerative Colitis
- VernacularTitle:Regulating Effects of Different Moxibustion Therapies on Bax and Bcl-2 in Rats with Ulcerative Colitis
- Author:
Huirong LIU
;
Shuang ZHOU
;
Xiaomei WANG
;
Huangan WU
;
Xuejun CUI
- Publication Type:Journal Article
- Keywords:
Moxibustion Therapy;
Colitis,Ulcerative;
Genes,bcl-2;
Gene Expression;
Rats
- From:
Journal of Acupuncture and Tuina Science
2010;08(3):174-180
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the improving effects of different moxibustion therapies on colonic injury in rats with ulcerative colitis (UC), and on the expression of Bcl-2 and Bax mRNA.Methods: Rats model of UC was made with immune methods and local stimulation. The models were treated with different moxibustion therapies 14 times, and then the expression of Bcl-2 and Bax mRNA were tested in the colonic tissue with fluorescent quantitative PCR. Results: (1)General scores of colon and histologic scores of injury were improved in all treatment groups except the model group (P＜0.05, P＜0.01); (2) The expression of Bcl-2 mRNA in the model rats of UC was higher than that in the normal rats (P＜0.01), whereas Bax lower (P＜0.01); (3) After treatments, the expression of Bcl-2 mRNA was lower in the herb cake-partitioned moxibustion, ginger-partitioned moxibustion, and garlic-partitioned moxibustion groups than that in the model group (P＜0.05, P＜0.01), and Bax mRNA in the herb cake-partitioned and ginger-partitioned groups was higher (P＜0.05). Conclusion: Herb cake-partitioned moxibustion, ginger-partitioned moxibustion, garlic-partitioned moxibustion, and mild moxibustion could improve the pathological injury in UC rats. Herb cake-partitioned moxibustion, ginger-partitioned moxibustion, and garlic-partitioned moxibustion could inhibit the expression of Bcl-2 mRNA and Bax mRNA in the colonic tissue of UC rats to regulate the ratio of Bcl-2/Bax mRNA.
