Effects of IL-24 delE5 on human leukemia cell line K562
10.3760/cma.j.issn.1009-9921.2010.03.001
- VernacularTitle:白细胞介素-24 delE5对人类白血病细胞系K562的抑制作用
- Author:
Lin WANG
;
Xiaotong MA
;
Chengya DONG
;
Fang ZHANG
;
Yongjuan DUAN
;
Binxia YANG
;
Yongmin LIN
- Publication Type:Journal Article
- Keywords:
mda-7/IL-24;
Spliceosomes;
Gene therapy;
K562 cells;
Cell cycle
- From:
Journal of Leukemia & Lymphoma
2010;19(3):129-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antitumor activity of IL-24 delE5 in human leukemia cell line K562. Methods The expression of mda-7/IL-24 and its splice variant induced by TPA in leukemic cell lines, U937 and HL-60, was evaluated. The effects of IL-24 delE5 in K562 on cell proliferation, colony-forming ability, cell cycle, apoptosis, and tumor growth in vivo by using MTr assay, colony forming assay, flow cytometry, Annexin-V/PI and tumor xenograft models in nude mice were assessed. Meantime, the effects of IL-24 delE-5 and mda-7/IL-2A were compared. Results The expression of IL-24 dciE5 was detected in differentiated U937 and HL-60 cells. Transfection with IL-24 delE5 significantly reduced tumor cell viability, inhibited colony formation. Comparing with the control, G_0/G_1 stage add from (24.46±3.99) % to (42.69±3.04) %, caused cell cycle arrest in G_0/G_1 stage and significantly inhibited the growth of K562 transplantation tumor. No significant differences in the aforementioned antileukemia characteristics between IL-24 delE5 and mda-7/IL-24 was found. Conclusion Similar with mda-7/IL-24, IL-24 delE5 can efficiently inhibit the proliferation of K562 in vitro and in vivo, probably through induction of G_0/G_1 cell cycle arrest.
