Effect of Acupuncture on Expressions of InsR-β mRNA and Protein in the Liver of Rats with Insulin Resistance
10.1007/s11726-009-0196-3
- VernacularTitle:Effect of Acupuncture on Expressions of InsR-β mRNA and Protein in the Liver of Rats with Insulin Resistance
- Author:
Jian SUN
;
Nenggui XU
;
Wei YI
;
Zhen JIA
;
Zhonghua YANG
;
Xuejun CUI
- Publication Type:Journal Article
- Keywords:
Insulin Resistance;
Acupuncture Therapy;
Receptor,Insulin;
Rats
- From:
Journal of Acupuncture and Tuina Science
2009;7(4):196-199
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of acupuncture on the expressions of insulin receptor β (InsR-β) mRNA and protein in the liver of experimental rats with insulin resistance (IR). Method: Twenty-four rats were randomly divided into 3 groups, control group (n=8),model group (n=8) and acupuncture group (n=8). Rats in the control group were fed with conventional food, and the other rats were induced into insulin resistance model with high fat-sugar-salt food. Once model was induced successfully, rats in the control group were fed with conventional food continually, rats in the model group were fed with high fat-sugar-salt food continually, and rats in the acupuncture group were fed with high fat-sugar-salt food, and treated with acupuncture for 2 weeks. The expression of InsR-β mRNA was detected by real-time fluorescent quantitative RT-PCR, and the expression of InsR-β protein was detected by Western blot. Result: Expressions of InsR-β mRNA and protein in the model group were significantly lower than those in the control group (P<0.01), and those in the acupuncture group were higher than those in the model group (P<0.01, P<0.05). The expressions of InsR-β mRNA and protein between the acupuncture and control group had no significant difference. Conclusion:Acupuncture treatment can increase the expressions of InsR-β mRNA and protein in IR rats'liver to improve insulin resistance.
