Induction of phenotype variance of naive T cell derived in vitro from mesenchymal stem cells
10.3760/cma.j.issn.1009-9921.2009.01.001
- VernacularTitle:间充质干细胞诱导体外分化来的初始T细胞表型变化的实验研究
- Author:
Jiangang JIN
;
Kai FENG
;
Bingyi SHI
;
Hu CHEN
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cell;
Naive T cells;
lmmunomodulatory
- From:
Journal of Leukemia & Lymphoma
2009;18(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective Donor derived naive T cells initiated GVHD by contacting with,mesenchymal stem cells(MSC)have been used to prevent or treat graft-versus-host disease(GVHD).although we stiff puzzle about its mechanisms.Observe the effect of MSC on phenotypes of Naive T cell to study the mechanism of MSC immunomodulation. Methods After 3 passages, MSC Was incubated with Naive T cell differentiated from COrd blood CD+34 cells in vitro.Then the variances of naive T cell phenotypes were analyzed by flow cytometric.Results CD+8 T cells were relatively increased after 7 days co-culture with allogenic MSC when compared to control:(35.9±6.3)%VS(18.4±4.5)%.CD+8 CD+3 cells also showed the same trend (27.6±2.8)%vs(15.2±3.1)%.Conclusion MSC may partly reduce the incidence of GVHD by increase of CD+8 naive T cell.The result may provide new clue to explain immunoregulatory mechanism of MSC.